Plasmid_Backbone

Part:BBa_K2205026:Design

Designed by: Lais Takiguchi   Group: iGEM17_Newcastle   (2017-10-23)


pSB1A51 - Promoter Library Screening Plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 111
    Illegal SpeI site found at 119
    Illegal PstI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 96
    Illegal SpeI site found at 119
    Illegal PstI site found at 979
    Illegal NotI site found at 102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 96
    Illegal BamHI site found at 173
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 111
    Illegal SpeI site found at 119
    Illegal PstI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 111
    Illegal SpeI site found at 119
    Illegal PstI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2060
    Illegal SapI.rc site found at 191


Design Notes

Design Features.


Source

Improved from BBa_J61002.

References